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Fraser Broth Base Dehydrated Culture Media

Product Descriptio n  
 
Fraser Broth Base is used for the selective enrichment of Listeria monocytogenes.    
 
Available in the following sizes:
500g, 2.5K, 10K

Our primary line of Dehydrated Culture Media is made with the best ingredients from BSE free countries. We provide dehydrated culture media in a wide array of formulas that follow FDA-BAM, USDA MLG, ISO, or your specified criteria.

SKU: M160

  • Technical Information
  • SDS
  • Certification of Quality
Cli   ck    he re for technical information for M160.

Fraser Broth Base
PRODUCT INFORMATION

DESCRIPTION: Fraser Broth Base was first described by Fraser and Sperber¹ and is used for the rapid detection of Listeria from food and environmental samples. Peptones and yeast extracts supply necessary growth nutrients. The phosphate salts supply buffering capacity. The addition of esculin and ferric ammonium citrate detect esculin hydrolyzing bacteria (Listeria). The broth is made selective by the addition of antibiotics and other agents. Lithium chloride inhibits Enterococcus sp. which also hydrolyze esculin. Nalidixic acid inhibits the growth of gram-negative microorganisms and acriflavine inhibits gram-positive microorganisms.

Formula* per Liter

  Pancreatic Digest of Casein........................................ 5.0g

  Peptic Digest of Animal Tissue................................... 5.0g

  Beef Extract................................................................... 5.0g

  Yeast Extract................................................................. 5.0g

  Sodium Chloride.......................................................... 20.0g

  Disodium Phosphate................................................... 9.6g

  Monopotassium Phosphate........................................ 1.35g

  Esculin.......................................................................... 1.0g

  Acriflavine………………………………………………….0.024g

  Nalidixic Acid………………………………………………0.020g

  Lithium Chloride........................................................... 3.0g

 

Fraser Broth Supplement:

      Per 10 mL Vial

       Ferric Ammonium Citrate....................... 0.5 g

 

*Adjusted and/or supplemented as required to meet performance criteria.

Final pH: 7.2 ± 0.2 at 25ºC

 Click       here  to access the Safety Data Sheet for Fraser Broth Base.    
Contact us to request a Certificate of Quality. Be sure to include the Product Number and Lot Number.

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Anaheim, California 92801

Office: (714)773-1726 
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