XLT4 Agar
PRODUCT INFORMATION

DESCRIPTION: Numerous media have been developed for isolating and differentiating enteric pathogens. Overgrowth of nuisance or contaminating organisms can be a major problem when recovery of a specific organism or species is desired. This is particularly true for Salmonella isolation media where overgrowth of Proteus, Providencia and Pseudomonas can dramatically interfere with the detection and isolation of Salmonella. In 1990, Miller and Tate described a new medium, XLT4 Agar, for isolating Salmonella. The authors established the selectivity of XLT4 Agar using pure cultures of a variety of enteric organisms. In follow-up studies, Miller and Tate reported that XLT4 Agar significantly improved the recovery of non-typhi Salmonella from chicken and farm environmental drag-swab samples.

Formula* per Liter

Proteose Peptone................................................. 4.6g

Yeast Extract.......................................................... 3.0g

L-Lysine................................................................. 5.0g

Xylose.................................................................. 3.75g

Lactose.................................................................. 7.5g

Saccharose........................................................... 7.5g

Ferric Ammonium Citrate...................................... 0.8g

Sodium Thiosulfate............................................... 6.8g

Sodium Chloride................................................... 5.0g

Agar...................................................................... 18.0g

Phenol Red......................................................... 0.08g

Total................................................................. 59.0g

*Adjusted and/or supplemented as required to meet performance criteria.

Final pH: 7.4 ± 0.2 at 25ºC